Is there a radical test
Modern laboratory diagnostics enable both the early detection of diseases and timely therapy and thus contribute to your health.
A simple blood test can provide assurance about the balance between free radical oxidative exposure and antioxidant potential:
d-ROMs test:Oxidative stress test.
The d-ROMs test shows the level of exposure to free radicals and provides information about the general health status of the body. A status that is largely dependent on the rhythm of biological oxidation. The values of the d-ROMs test are an unmistakable "mirror" of oxidative processes and provide information about the rate of the physiological aging process.
BAP test:Biological antioxidant potential.
The BAP value provides information about the state of the body's own antioxidant defense system against free radicals. The test shows how well the body is able to render free radicals harmless in order to protect itself from damage. At the same time, a possible lack of antioxidants is determined.
© Bioradicals GmbH 2008
d-ROMs test: Free radicals, mostly oxygen radicals, react with organic molecules in the body, creating so-called ROMs (Reactive Oxygen Metabolites) as by-products. ROMs are relatively more stable than their precursors, the free radicals, and have a medium oxidative potential. A main component of the ROMs are hydroperoxides, which, due to their special properties, can be determined with the d-ROMs test. In the presence of a transition metal, which in turn acts as a catalyst, these generate free radicals, which are "captured" with a chromogen for measurement. On the basis of the available scientific literature, the d-ROMs test, in the context of its determination, was tested in almost all relevant fields of human medicine.
BAP test: The BAP test allows a reliable determination of the antioxidative potential, the part of the plasma barrier that is directly affected by oxidation, due to its reduction / oxidation potentials under "physiological" conditions.
In the blood, the so-called antioxidant plasma barrier guarantees a defense against harmful attacks by reactive species and especially against free radicals. This barrier has both exogenous - z. B. vitamin C, E, carotenoids, bioflavonoids etc. - as well as endogenous - z. B. proteins, bilirubin, uric acid, cholesterol, GSH etc. - components. Each of these components has its own antioxidant potential (or capacity). Depending on their reduction / oxidation potential, they can counteract attacks by reactive species in different ways. Such a capacity is linked to the ability of individual components from the plasma barrier to release so-called reduction equivalents to free radicals - i.e. either electrons or H atoms - and thus to prevent the dissociation of H atoms from biomolecules, which trigger a radical chain reaction. In fact, any violation of the “antioxidant plasma barrier” can result in oxidative damage to cells and tissue.
- Franzini A, Luraschi P, Pagani A. Direct measurement of reactive oxygen metabolites in blood serum: analytical assessment of a novel method Biochimica Clinica. 1996. Suppl. 1 / 5-6: 89.
- Alberti A. The d-ROMs test. Model and chemical basis I.Co.C.E.A. - National Council of Research. Research Area. Bologna, Italy. 1997.
- Verde V, Fogliano V, Ritieni A, Maiani G, Morisco F, Caporaso N. Use of N, N-dimethyl-p-phenylenediamine to evaluate the oxidative status of human plasma. Free Radic Res. 2002. 36 (8): 869-873.
- Iamele L, Fiocchi R, Vernocchi A. Evaluation of an automated spectrophotometric assay for reactive oxygen metabolites in serum. Clin Chem Lab Med. 2002. 40 (7): 673-676.
- Lucchi L, Iannone A, Bergamini S, Stipo L, Perrone S, Uggeri S, Gatti V, Ferrari F, Tomasi A, Albertazzi A. Comparison between hydroperoxides and malondialdehyde as markers of oxidative acute injury during hemodialysis. Artificial organs. 2005. 29: 832.
- Iorio EL, Cinquanta L, Pisano R. A diagnostic algorithm for the oxidative stress. Italian Journal of Laboratory Medicine. 2005. 1 (3): 137.
- Cighetti G, Paroni R, Marzorati S, Borotto E, Giudici R, Magnanimi G, Iapichino G. Evaluation of oxidative stress in serum of critically ill patients by a commercial assay and gas chromatography-mass spectrometry. Clin Chem. 2005. 51 (8): 1515-1517.
- Rosolia A, Di Leo T, Iorio EL, Lipartiti T. The d- ROMs test automation: analytical performances and clinical applications. Italian Journal of Laboratory Medicine. 2005. 1 (3): 172.
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